Date of Completion
nanodisc, NMR, DMPC lipids, nanoscale bilayer system, TEM, membrane scaffold protein, beta-barrel, transmembrane helix, MOSP, TprC
Dr. Olga Vinogradova
Dr. Nathan Alder
Dr. Andrew Wiemer
Dr. Arlene Albert
Dr. Debra Kendall
Field of Study
Structural Biology and Biophysics
Doctor of Philosophy
Several membrane proteins, comprising of either α-helical or β-barrel structures have been successfully studied in vitro using several membrane mimetic systems. Nanodiscs, a new class of discoidal nanoscale lipoprotein complex have been used extensively in the present study to investigate different membrane proteins. We begin by delineating the development of small (D7) nanodiscs through its rigorous characterization and demonstrate advantages in solution NMR applications. In addition, we show the development of an on-column method to generate nanodiscs through the selective use of variable protein tags. D7 discs have been further used to visualize the downstream phosphorylation events of activated integrin β3 through Src kinase. The C-terminal domains, TprC and MOSPC, two β-barrel membrane porins, were shown to trimerize when visualized through negatively stained transmission electron microscopy images in nanodiscs. The periplasmic and membrane conformers of native MOSP in Treponema denticola was established along with the partial identification of their multimeric complexes through a series of immuno-chemical experiments. Also discussed are two specific studies which revisit previously published papers where, (a) the direction of the cytoplasmic tail of integrin β3 bound to the SH3 domain of Src kinase was shown to be in the reverse orientation w.r.t the published crystal structure; and (b) PLIC proteins, [Protein Linking the cell membrane (IAP/CD47) to the Cytoskeleton] was shown to be involved in proteasomal degradation with no binding ability to the cytoplasmic tail of CD47. Small angle X-ray scattering studies of BTN3A1, an immune modulator, revealed the rearrangement of the intracellular cytoplasmic B30.2 domain with respect to the juxta membrane region in the presence of a phosphoantigen HMBPP. Lastly, a novel finding is briefly described involving orthlogs of TamB, a component of the transloaction and assembly module system, comprising of the TamA-TamB complex in proteobacteria, interacting with BamA (Beta-barrel assembly machinery protein A) in bacterial diderms where TamA is absent.
Puthenveetil, Robbins J., "Structure-Function Investigation of Membrane Proteins Using Nanodiscs" (2017). Doctoral Dissertations. 1351.
Available for download on Monday, October 09, 2017