Document Type

Article

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

We have assessed the utility of five new long-wavelength fluorescent voltage-sensitive dyes (VSD) for imaging the activity of populations of neurons in mouse brain slices. Although all the five were capable of detecting activity resulting from activation of the Schaffer collateral-CA1 pyramidal cell synapse, they differed significantly in their properties, most notably in the signal-to-noise ratio of the changes in dye fluorescence associated with neuronal activity. Two of these dyes, Di-2-ANBDQPQ and Di-1-APEFEQPQ, should prove particularly useful for imaging activity in brain tissue and for combining VSD imaging with the control of neuronal activity via light-activated proteins such as channelrhodopsin-2 and halorhodopsin.

Comments

Brain Cell Biol. Author manuscript; available in PMC 2013 May 24.

Published in final edited form as: Brain Cell Biol. 2008 December; 36(0): 157–172.

Published online 2009 February 14. doi: 10.1007/s11068-009-9039-x

PMCID: PMC3663446 NIHMSID: NIHMS164890

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